Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Welin, M.; Skovgaard, T.; Knecht, W.; Zhu, C.; Berenstein, D.; Munch-Petersen, B.; Piskur, J.; Eklund, H.
    Structural basis for the changed substrate specificity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D (2005), FEBS J., 272, 3733-3742.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.145 expression of the wild-type and mutant enzymes in enzyme-deficient Escherichia coli strain KY895 Drosophila melanogaster

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.145 purified recombinant mutant N64D in complex with dT or dTTP, counter diffusion and vapour diffusion methods, 20 mg/ml enzyme with 10 mM dT, is mixed with cyrstallization solution containing 0.15 M MES, pH 6.5, 0.3 M lithium sulfate, and 27,5% w/v PEG 2000 monomethylether, or 10 mg/ml enzyme with 5 mM dTTP is mixed with 0.1 M MES, pH 6.5, 0.16 M lithium sulfate, and 25% v/v PEG 2000 monomethylether, 2 weeks, 15°C, X-ray diffraction structure determination and analysis at 3.1 A and 2.2 A resolution, respectively Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.145 additional information expression of the enzyme in enzyme-deficient Escherichia coli strain KY895 confers resistnce against 3'-azido-2',3'-didehydro-3'-deoxythymidine at more than 0.1 mM Drosophila melanogaster
2.7.1.145 N45D site-directed mutagenesis, decreased activity with natural substrates Drosophila melanogaster
2.7.1.145 N45D/N64D decreased activity with natural substrates, decreased feedback inhibition by dTTP compared to the wild-type enzyme Drosophila melanogaster
2.7.1.145 N64D site-directed mutagenesis, highly decreased activity with natural substrates, decreased feedback inhibition by dTTP compared to the wild-type enzyme Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.145 dTTP competitive versus ATP, feedback inhibition, binding at the active site, binding structure of mutant N64D, modelling Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.145 0.0009
-
 2'-deoxycytidine recombinant mutant N45D Drosophila melanogaster
2.7.1.145 0.0009
-
 2'-deoxythymidine recombinant mutants N45D Drosophila melanogaster
2.7.1.145 0.0111
-
 3'-azido-2',3'-didehydro-3'-deoxythymidine recombinant mutant N64D Drosophila melanogaster
2.7.1.145 0.0117
-
 3'-azido-2',3'-didehydro-3'-deoxythymidine recombinant mutant N45D Drosophila melanogaster
2.7.1.145 0.0232
-
 2'-deoxythymidine recombinant mutant N64D Drosophila melanogaster
2.7.1.145 0.118
-
 2'-deoxycytidine recombinant mutant N64D Drosophila melanogaster
2.7.1.145 0.119
-
 2'-deoxyadenosine recombinant mutant N45D Drosophila melanogaster
2.7.1.145 0.412
-
 2'-deoxyguanosine recombinant mutant N45D Drosophila melanogaster
2.7.1.145 3.82
-
 2'-deoxyadenosine recombinant mutant N64D Drosophila melanogaster
2.7.1.145 20.35
-
 2'-deoxyguanosine recombinant mutant N64D Drosophila melanogaster

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.145 Mg2+
-
 Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.145 ATP + 2'-deoxyadenosine Drosophila melanogaster
-
 ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine Drosophila melanogaster
-
 ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyguanosine Drosophila melanogaster
-
 ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine Drosophila melanogaster
-
 ADP + 2'-deoxythymidine 5'-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.145 Drosophila melanogaster
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.145 additional information
-
-
 Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.145 ATP + 2'-deoxyadenosine
-
 Drosophila melanogaster ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine
-
 Drosophila melanogaster ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyguanosine
-
 Drosophila melanogaster ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine
-
 Drosophila melanogaster ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine substrate binding structure of mutant N64D Drosophila melanogaster ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine
-
 Drosophila melanogaster ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + aciclovir
-
 Drosophila melanogaster ADP + aciclovir phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.145 dNK
-
 Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.145 0.03
-
 3'-azido-2',3'-didehydro-3'-deoxythymidine recombinant mutant N45D Drosophila melanogaster
2.7.1.145 0.037
-
 3'-azido-2',3'-didehydro-3'-deoxythymidine recombinant mutant N64D Drosophila melanogaster
2.7.1.145 0.12
-
 2'-deoxyguanosine recombinant mutant N64D Drosophila melanogaster
2.7.1.145 0.4
-
 2'-deoxyadenosine recombinant mutant N64D Drosophila melanogaster
2.7.1.145 0.82
-
 2'-deoxythymidine recombinant mutant N64D Drosophila melanogaster
2.7.1.145 1.3
-
 2'-deoxyguanosine recombinant mutant N45D Drosophila melanogaster
2.7.1.145 1.6
-
 2'-deoxycytidine recombinant mutants N45D and N64D Drosophila melanogaster
2.7.1.145 1.83
-
 2'-deoxyadenosine recombinant mutant N45D Drosophila melanogaster
2.7.1.145 2.6
-
 2'-deoxythymidine recombinant mutant N45D Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.145 ATP
-
 Drosophila melanogaster

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.145 0.0047
-
 dTTP uncompetitive versus 2'-deoxythymidine, recombinant wild-type enzyme Drosophila melanogaster
2.7.1.145 0.0163
-
 dTTP competitive versus ATP, recombinant wild-type enzyme Drosophila melanogaster
2.7.1.145 0.52
-
 dTTP uncompetitive versus 2'-deoxythymidine, recombinant mutant N64D Drosophila melanogaster
2.7.1.145 0.829
-
 dTTP competitive versus ATP, recombinant mutant N64D Drosophila melanogaster